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KMID : 1123920050190030722
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Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 3 p.722 ~ p.728
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Growth Inhibition of Human Lung Carcinoma Cells by ${\beta}>-lapachone$ through Induction of Apoptosis
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Choi Yung-Hyun
Choi Byung-Tae
Lee Yong-Tae
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Abstract
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The DNA topoismerase I inhibitor , the product of a lapacho tree (Tabebuia avellanedae) from South America, activates a novel apoptotic response in a number of cell lines. In the present report, we investigated the effects of on the growth of human lung in human non-small-cell-lung-cancer A549 cells. Upon treatment with , a concentration-dependent inhibition of cell viability and cell proliferation was observed as measured by hemocytometer counts and MTT assay. The cells developed many of the hallmark features of apoptosis, including membrane shrinking, condensation of chromatin and DNA fragmentation. These apoptotic effects of in A549 cells were associated with a marked induction of pro-apoptotic Bax expression, however the levels of anti-apoptotic Bcl-2 expression were decreased in a dose-dependent manner. Accordingly, elevated amount of cyclin-dependent kinase inhibitor p21 expression accompanied by up-regulation of tumor suppressor p53 was observed. By RT-PCR analyses, decrease in gene expression level of telomerase reverse transcriptase and telomeric repeat binding factor were also observed. Thus, these findings suggest that may be a potential anti-cancer therapeutics for the control of human lung cancer cell model.
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KEYWORD
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lung carcinoma, apoptosis, Bax, Bcl-2
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